This edition maintains emphasis on basics, while updating the examples to include more recent results. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of time resolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. Glossaries are provided for commonly used acronyms and mathematical symbols.
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