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Rapid Cycle Real-Time PCR 在线电子书 pdf 下载 txt下载 epub 下载 mobi 下载 2024

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G. Burg 作者

Springer

译者

2001-2-15 出版日期

408 页数

USD 99.95 价格

Spiral-bound

丛书系列

9783540667360 图书编码

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Rapid Cycle Real-Time PCR 在线电子书 epub 下载 mobi 下载 pdf 下载 txt 下载 2024

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Rapid Cycle Real-Time PCR 在线电子书 pdf 下载 txt下载 epub 下载 mobi 在线电子书下载

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The first comprehensive treatise on Rapid Cycle Real-Time PCR. With amplification times of 15-30 minutes of on-line detection and analysis, nucleic acid quantification of mutation analysis finally becomes a routine, powerful and rapid method. Focusing primarily on the LightCycler, an instrument that combines Rapid Cycle PCR with fluorescent monitoring, this technology provides convenient analysis by melting temperatures. PCR products can be identified by product Tm, and single base mismatches can easily be genotyped by probe Tm. Methods chapters detail the theory behind quantification of mutation analysis; the design of synthesis of fluorescent hybridization probes of the preparation of template DNA. Application chapters apply nucleid acid quantification to infectious organisms of intracellular messengers and mutation detection to somatic of acquired mutations.

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