Transmembrane Signaling Protocols 在线电子书 pdf 下载 txt下载 epub 下载 mobi 下载 2024

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Ali, Hydar (EDT)/ Haribabu, Bodduluri (EDT) 作者

Humana Pr Inc

译者

2006-2 出版日期

376 页数

$ 157.07 价格

HRD

丛书系列

9781588295460 图书编码

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发表于2024-11-07

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Transmembrane Signaling Protocols 在线电子书 epub 下载 mobi 下载 pdf 下载 txt 下载 2024

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This fully updated and expanded second edition of Transmembrane Signaling Protocols demonstrates the use of such techniques as single cell imaging, bioluminecence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. The protocols follow the successful Methods in Molecular Biology(t) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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