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Directed Enzyme Evolution

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Arnold, Frances Hamilton (EDT)/ Arnold, Frances Hamilton/ Georgiou, George (EDT) 作者
Humana Press
译者
2003-05-16 出版日期
370 页数
USD 99.50 价格
Hardcover
丛书系列
9781588292865 图书编码

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Directed Enzyme Evolution 在线电子书 epub 下载 mobi 下载 pdf 下载 txt 下载 2024

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Directed Enzyme Evolution 在线电子书 pdf 下载 txt下载 epub 下载 mobi 下载 2024



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Directed Enzyme Evolution 在线电子书 图书描述

Seasoned practitioners from many leading laboratories describe their best readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. An accompanying volume, Directed Evolution Library Creation: Methods and Protocols (ISBN 1-58829-285-1), describes readily reproducible methods for the creation of mutated DNA molecules and DNA libraries.

Copy for Both Volumes

Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function.

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