Restriction Endonucleases 在线电子书 pdf 下载 txt下载 epub 下载 mobi 下载 2024

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Pingoud, Alfred M. (EDT) 作者

Springer Verlag

译者

出版日期

469 页数

$ 326.57 价格

HRD

丛书系列

9783540205029 图书编码

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发表于2024-11-11

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Restriction Endonucleases 在线电子书 epub 下载 mobi 下载 pdf 下载 txt 下载 2024

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Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems ), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.

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