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Pcr Cloning Protocols 在线电子书 pdf 下载 txt下载 epub 下载 mobi 下载 2024

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Chen, Bing-Yuan (EDT)/ Janes, Harry W. 作者

Humana Pr Inc

译者

2002-4 出版日期

453 页数

$ 213.57 价格

HRD

丛书系列

9780896039698 图书编码

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Pcr Cloning Protocols 在线电子书 epub 下载 mobi 下载 pdf 下载 txt 下载 2024

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PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

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