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Gene Cloning and Manipulation 在线电子书 pdf 下载 txt下载 epub 下载 mobi 下载 2025

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Howe, Christopher 作者

Cambridge Univ Pr

译者

2007-5 出版日期

276 页数

$ 158.20 价格

HRD

丛书系列

9780521817936 图书编码

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Gene Cloning and Manipulation 在线电子书 epub 下载 mobi 下载 pdf 下载 txt 下载 2025

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Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

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