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High Throughput Protein Expression and Purification

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Doyle, Sharon A 編 作者
Humana Press
譯者
2008-10-20 出版日期
334 頁數
USD 99.00 價格
Hardcover
叢書系列
9781588298799 圖書編碼

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High Throughput Protein Expression and Purification 在線電子書 epub 下載 mobi 下載 pdf 下載 txt 下載 2024

High Throughput Protein Expression and Purification 在線電子書 epub 下載 pdf 下載 mobi 下載 txt 下載 2024

High Throughput Protein Expression and Purification 在線電子書 pdf 下載 txt下載 epub 下載 mobi 下載 2024



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High Throughput Protein Expression and Purification 在線電子書 pdf 下載 txt下載 epub 下載 mobi 在線電子書下載

High Throughput Protein Expression and Purification 在線電子書 圖書描述

Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology(t) series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.

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