Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the 'two-component system'. The sections include: Structural Approaches; Reconstitution of Heterogeneous Systems; Intracellular Methods and Assays; and, Genome-Wide Analyses of Two-Component Systems. It presents detailed protocols and includes troubleshooting tips.
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