Completely revised, updated, and expanded, the third edition of the best-selling Molecular Biotechnology: Principles and Applications of Recombinant DNA covers the underlying scientific principles and deals extensively with the many industrial, agricultural, pharmaceutical, and biomedical applications of recombinant DNA technology. The latest edition offers greatly expanded coverage of directed mutagenesis and protein engineering, therapeutic agents, and genetic engineering of plants. Updated chapters reflect recent developments in biotechnology and the societal issues related to it, such as cloning, gene therapy, and patenting and releasing genetically engineered organisms. Over 500 figures, including 200 that are new in this edition, illustrate all key concepts. "Milestones" summarize important research papers in the history of biotechnology and their effects on the field. As with previous editions, the authors clearly explain all concepts and techniques to provide maximum understanding of the subject, avoiding wherever possible confusing scientific jargon and excessive detail. Each chapter concludes with a summary, references, and review questions. Ideally suited as a text for third- and fourth-year undergraduates as well as graduate students, this book is also an excellent reference for health professionals, scientists, engineers, or attorneys interested in biotechnology. New in the Third Edition Significantly updated to reflect the advances of the past five years Various recent examples replace those from the mid-1990s and earlier Over 200 new figures illustrate the added concepts and principles Covers the major advances since publication of the previous edition